Sunday, 24 March 2019

Integration and Autonomy in Brain Organoids Holistic Ethics and the Study of Human Brain Fragments


by Denis Larrivee & M. Farisco

Background and Thesis: Philosophy of Science, Metaphysics of Parts, and Privileging the Whole
Can a brain organoid be considered a whole? Do brain properties confer moral compulsion in isolation? Can brain fragments acquire normative status from their relation to the whole brain/individual? These are several of an array of questions emerging from the intersection of the metaphysical domain of parts and wholes and the domain wherein the human brain is normatively privileged. Such questions are uniquely raised by current research into human brain organoids, three-dimensional tissue culture preparations of human brain fragments, hence parts of whole brains. Classically, extending backward to Aristotle at least, the metaphysical question traces its origin to the recognition of the unity and independence of individual things. This understanding views the universe to be individuated, composed of entities that are embedded in the framework of space-time. Definitionally, entities are such because they a) have a unique spatio-temporal location, 2) are the subject of the predication of properties, and 3) are distinguished by qualitative properties from all other entities [Esfeld 2004]. Parts thus emerge as a subdomain not coextensive with the entity. This, still dominant, perspective is of significant contemporary interest in philosophy of science circles in so far as how physical composition is conceived, where notions like emergence and reductionism, or the constitution of systems, are broadly debated across scientific disciplines [Gillett 2016]. For brain organoid cultures, the metaphysical question intersects with normative issues due to the privileging accorded to human beings, and, by extension, to their brains and nervous systems. Aristotelian thinking, for example, denies that a hand can be so regarded when it no longer functions for the good of the whole individual. That the view we select on part, property, and whole critically determines the ethical practice we adopt in organoid use will be argued here.

Figure 1A
Nextbigfuture.com


Figure 1B
Sciencemag.org

Introduction: Scope of Contending Issues
Brain organoids are a readily available experimental source of neural tissue made from human pluripotent stem cells (Figure 2A, B). Because they are developed from human cells they possess a full complement of the human genetic repertoire; they are expected, therefore, to closely mimic physical properties of the brain’s neural operation. Their increasing sophistication and wide ranging experimental scope (Figure 3), including likely attempts to develop higher order properties, raise the possibility that they will soon pose significant ethical issues.
Which properties, how much these properties resemble those of humans, and which level of brain operation they reflect are matters typically impinging on their ethical valuation. However, because they are observed in tissue fragments, it is uncertain how such normative qualifications may apply.

Figure 2: Pluripotent stem cell production. Somatic cells from ectoderm are transfected
with Sox (Yamanaka) transcription factors to induce neural differentiation.

Functionalism: Can a Property be Privileged Alone?
Contemporary notions of brain properties are most often equated with functions [Levin 2018], where a function denotes a mental capacity. Functionalist models prevail, for example, in conceptions about the mind, like Extended Mind Theory (EMT), that equate the reality of the mind with the mind’s functioning Levy 2011], e.g., recollection, and that include operations conducted beyond the brain. Hence, functionalist models challenge the metaphysical reality of ‘wholes’, and so also value contingency in discrete entities alone. Accordingly, functionalist models tend to a permissive praxis in organoid use. A classical, individuated approach human brain, by contrast, views properties as existing only when present in a source. Properties, in this understanding do not predicate in isolation. Consistent with this view properties in nature emerge from systemic realities, like the embodied brain. Indeed, living systems appear to characterize all of natural reality, and to embrace mental function, which does not exist in their absence. This suggests that organoids must constitute systemic entities in their own right, in order to acquire a normative status that would preclude experimental manipulation.

Figure 3: Brain Organoids can be used for a wide variety of experimental purposes. 



Integration: Can a Part Acquire Normative Status?
Organoids, however, are most easily understood as parts of organisms and so possess only some, but not all, properties that accrue to living systems. This organismal perspective raises the question of whether organoids can constitute lesser entities, with quantitatively smaller or qualitatively different subsets of properties from those of whole organisms. That is, can a human brain organoid constitute a whole according to criteria used for a human organism? This question concerns two issues: first, whether a subset of properties constitute a viable whole, a philosophical issue significant for its use in the context of death determinations for humans, and second, whether a subset of human properties can be regarded as human. The first question is anchored in the particular notion of integration used, like that formulated by the 1984 Swedish Committee on Death Criteria that is premised on coherency and coordination [Swedish Committee on Death and Dying 1984]. Clinical ethics for death determinations conclude that in the absence of coherency and coordination a human being does not exist. By extension, organoids that are not integrated, according to this definition, would not exist as integral wholes. Conversely, should this notion of integration obtain, can the presence of a subset of properties then distinguish the organoid as human? Classically, the absence of one or more human properties would distinguish an organoid from a human entity [Esfeld 2004]; according to this understanding, only a full complement of human, organismal properties (which predicate from the whole) possess normative status, a state not achieved in organoids. By either reasoning research on organoids would be broadly permissible.

Autonomy: Which Properties Confer Normative Status?
Are there properties which are so distinctive as to be normatively valued? Some, like self consciousness, or at least sentience, seem so unique as to ethically preclude intervention. This perspective would suggest that some property subsets are ethically probative; hence, in these circumstances a classical understanding of distinct entities with a unique set of properties would appear inadequate to normatively distinguish an organoid from a human being. In this case both organoid and individual appear to be qualified by the same normative distinction; indeed, to be both human. The possession of a partial complement of higher order human properties seems unlikely, however; rather, higher order properties appear to occur only in unison. The ‘natural’ evidence suggests, in fact, that in the evolutionary patrimony living systems are purposed to autonomous existence [Mossio Moreno 2015], a circumstance requiring all properties together. Much evidence indicates that this purposing cannot occur by the nervous system in isolation, but requires a systemic embodied dimension to elicit them. Indeed, key organismal properties appear to emerge from the body’s association with the brain to generate higher order activity and to sustain autonomy [Damasio 2012]. Moreover, a consensus global state that is self identifying appears to lack sufficient representational content in the absence of the body, demonstrated, for example, in cases of sensory deprivation [Wiesel Hubel 1963]. Hence, organoids appear destined to remain parts, incapable of progressing to human wholes.

Caveats: Cloning and Property Proximity
Nonetheless, the human being remains specially privileged and the use of a full genetic complement seems to portend access to avenues of exploitation. Developmental paradigms, which address the many biological details yet unresolved, have the potential for manipulation of the whole human, a prospect Kant identified as morally offensive. Current experimental approaches that approximate cloning (akin to parthogenesis) [Kao et al 2010] and that initiate developmental trajectories equivalent to those undertaken for the whole human appear, thus, to be distinguished from the study of brain parts and will likely require close ethical oversight. The generation of whole systems, albeit over time, raise the corollary of property proximity and when a variant is sufficiently different to no longer be seen as human.

Figure 4: Cloning procedure from induced pluripotent stem cells.

This seems to suggest that while the ethical terrain of organoids may be governed by metaphysical principles on parts and wholes, which are revealed by their expression in the natural world, new insights on the nature of the whole will likely be required. Concepts like spatiotemporal trajectories, higher order integration, and integral development appear especially pertinent. That is, a new conception of the whole, and the relationship of the part to it, appears to be needed, one that is not merely static, but systemic, ontological, and dynamic.

Concepts

  • The development of ethical guidelines for organoid manipulation is intimately linked to the metaphysical concepts of parts and wholes and their relation to contingent value
  • However, a static conception of the whole and how higher order cognitive properties are integrated into it will likely require revision to a new systemic and autonomous account of the nature of integration
  • These lacunae may also need addressing by a spatio-temporal dynamic that underpins the metaphysical understanding of the whole.
  • Experimental manipulation infringing on such physically instantiated wholes may be deemed probative.


References
Esfeld M (2004). Quantum Entanglement and a Metaphysics of Relations. Studies Hist Phil Mod Phys 35: 601–617.
Gillet C (2016) Reduction and emergence in science and philosophy. Cambridge University Press, Cambridge.
Levin J (2018) Functionalism Stanford Encyc Phil
Levy N. (2011) Neuroethics and the Extended Mind. In Handbook for Neuroethics. Sahakian B, Illes J (eds) Oxford: Oxford University Press.
Kao et al (2010) Mice cloned from induced pluripotent stem cells (iPSCs) Biol Reprod 83(2):238-243.
Moreno A, Mossio M (2015) Biological autonomy: a philosophical and theoretical inquiry. Springer Publishing, Dordrecht
Wiesel TN, Hubel DH (1963) Single cell responses in striate cortex of kittens deprived of vision in one eye. J Neurophys 26:1003-1017
Swedish Committee on Defining Death (1984) The concept of death. Summary. Stockholm: Swedish Ministry of Health and Social Affairs.
Damasio A (2012) Self comes to mind: constructing the conscious brain. Pantheon Books, New York.

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